Title
Immunomodulation by Maharishi Amrit Kalash [MAK-4] in Mice

Publication
Journal of Applied Nutrition, Vol. 48, Nos. 1 and 2, pp. 10-21, 1996.

Authors
Ryoichi Inaba, PhD,* Haruo Sugiura, PhD,* Hirotoshi Iwata, PhD,* Hiroshi Mori, PhD,** and Takuji Tanaka, PhD.Ú

Conducted at
*Department of Hygiene, Gifu University School of Medicine, Gifu, Japan
**Department of Microbiology, Gifu Pharmaceutical University, Gifu, Japan
Ú First Department of Pathology, Gifu University School of Medicine, Gifu, Japan

Summary

The effects of ingestion of Maharishi Amrit Kalash-4 (MAK-4), one of the Ayurvedic food supplements, on immune function were evaluated in male A/He mice aged 7 weeks. Production of nitric oxide (NO) by peritoneal macrophages and proliferation of spleen cells stimulated by mitogens was examined in mice given MAK-4 by gastric intubation at the doses of 10, 50, and 100 mg/kg once a day for 20 days. Glucose consumption of peritoneal macrophages during incubation up to 72 hours at all doses of MAK-4 was significantly higher in the MAK-4 treated mice than in the control group (p<0.05). Activities of lactate dehydrogenase in the peritoneal macrophages were significantly increased in the MAK-4 treated mice at all doses (p<0.01). Macrophage production of NO stimulated by lipopolysaccharide in the MAK-4 treated mice at all doses was significantly increased (p<0.01). Stimulation indices both by concanavalin A (Con A) and phytohaemagglutinin in the MAK-4 treated groups at all doses were significantly higher than those of the control group (p<0.05). Splenocyte production of interleukin-2 (IL-2) stimulated by Con A in the MAK-4 treated mice at all doses was significantly increased (p<0.01). MAK-4 treated mice at the dose of 10 mg/kg had the highest IL-2 production by splenocytes. MAK-4 at any of the doses used did not enhance spontaneous NO production, spontaneous splenic lymphocyte proliferation, or spontaneous IL-2 production by splenocytes. These results indicate that gastric intubation of MAK-4 once a day at a dose of 10 mg/kg or more enhances not only macrophage function but also lymphocyte responsiveness in mice.

Please Note

MAK-4 is now called Amrit Kalash Nectar.


Disclaimer
The sole purpose of these articles is to provide information about the tradition of ayurveda. This information is not intended for use in the diagnosis, treatment, cure or prevention of any disease. If you have any serious acute or chronic health concern, please consult a trained health professional who can fully assess your needs and address them effectively. If you are seeking the medical advice of a trained ayurvedic expert, call or e-mail us for the number of a physician in your area. Check with your doctor before taking herbs or using essential oils when pregnant or nursing.