Anti-Oxidant and Antiplatelet Properties of Maharishi Amrit Kalash [MAK-4] in Hypercholesterolemic Rabbits

Ninth International Symposium on Atherosclerosis, Rosemont, IL, October 6-11, 1991, p. 188 (Abstract).

Rao V. Panganamala, Ph.D. and Hari M. Sharma, M.D., FRCP(C).

Conducted at
The Ohio State University, College of Medicine, Columbus, OH, USA


Platelet aggregation and oxidized lipids are considered important mediators of vascular injury leading to atherosclerosis. M-4, an herbal food supplement (MAPI Inc., Lancaster, MA) has been shown to be effective in preventing generation of reactive oxygen species in-vitro (IJCP 1:23-27, 1991). The experiments were carried out to evaluate the effectiveness of M-4 in preventing platelet aggregation and oxidation of lipids in hypercholesterolemic rabbits. Two groups of six rabbits (pair matched) were given a 1% cholesterol diet. The experimental group in addition was given 0.4% M-4 in the diet. At the end of the experiment (7 weeks), total cholesterol, plasma and hepatic TBARS, and platelet aggregation induced by ADP & collagen were compared between the two groups.

The results are

  Control Experimental
Total cholesterol (mg/dl) 1511 1003
Plasma TBARS (nmoles/ml) 5.38 ± 0.5 1.9 ± 0.3
Hepatic TBARS (nmoles/g tissue) 148 ± 16  83 ± 11
Platelet Aggregation % Transmittance Collagen (ug/ml) ADP (x 10-3)
  4.4        2.2 4.4      2.2
Control (n = 5) 48.6       30.0 34.0     21.0
Experimental (n = 4) 26.0        3.0
10.0      3.75

Results show that M-4 supplementation reduces plasma and hepatic lipid peroxidation, as well as platelet aggregation induced by collagen and ADP, in hypercholesterolemic rabbits.

Please Note

MAK-4 is now called Amrit Kalash Nectar.

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