Title
In Vivo Effect of Herbal Mixture MAK-4 on Antioxidant Capacity of Brain Microsomes

Publication
Biochemical Archives, Vol. 12, pp. 181-186, 1996.

Authors
Hari M. Sharma, Jae Y. Lee, Ellen M. Kauffman, and Atef N. Hanna.

Conducted at
Department of Pathology, College of Medicine, The Ohio State University, Columbus, OH 43210

Summary

There is increasing evidence that free radicals are linked to neurological disorders and aging. The herbal mixture Maharishi Amrit Kalash-4 (MAK-4) has antioxidant properties, as assessed by inhibition of low-density lipoprotein oxidation in vivo and in vitro. This study examined the in vivo effect of MAK-4 on lipid peroxidation and antioxidant protection capacity of the brain of Watanabe Heritable Hyperlipidemic (WHHL) rabbits. A group of 5 rabbits (controls) were fed normal rabbit chow, and a group of 6 rabbits were fed normal chow supplemented with 6% MAK-4 (w:w) for 6 months. Brain microsomes were then prepared and incubated in the presence or absence of either an enzymatic or nonenzymatic system for inducing lipid peroxidation; in the absence of either system, air-induced lipid peroxidation was measured. Lipid peroxidation was assessed by measuring thiobarbituric acid-reactive substances (TBARS). The baseline level of TBARS (nmoles malondialdehyde/mg microsomal protein) was significantly lower (p<0.05) in the rabbits fed MAK-4 (1.18 +/- 0.07 vs. 1.51 +/- 0.25 for controls). Also, the MAK-4 group showed significantly lower TBARS (p<0.05) after air-, enzymatic-, and nonenzymatic-induced lipid peroxidation (1.29 +/- 0.21, 1.27 +/- 0.16, and 2.91 +/- 0.79, respectively), as compared to controls (1.92 +/- 0.45, 2.28 +/- 0.26, and 12.85 +/- 0.61, respectively). These results indicate MAK-4 may yield increased antioxidant protection in the brain, and may therefore be useful in preventing or treating free radical-induced neurological disorders.

Please Note

MAK-4 is now called Amrit Kalash Nectar.


Disclaimer
The sole purpose of these articles is to provide information about the tradition of ayurveda. This information is not intended for use in the diagnosis, treatment, cure or prevention of any disease. If you have any serious acute or chronic health concern, please consult a trained health professional who can fully assess your needs and address them effectively. If you are seeking the medical advice of a trained ayurvedic expert, call or e-mail us for the number of a physician in your area. Check with your doctor before taking herbs or using essential oils when pregnant or nursing.